Immune response induced by a different combined immunization of HBsAg vaccine
- 生命科学－已发表论文 
Aims: To evaluate the immune responses induced by different combined immunizations of HBsAg protein vaccine ( P), recombinant vaccinia virus vaccine ( V) and DNA vaccine ( D). Methods: Balb/c mice were primed by one of the three HBsAg vaccines P, V or D and boosted by the same or another, thus nine immune combinations were constructed. Titers of anti-HBsAg IgG and their sub-isotypes were determined by ELISA. Specific cellular immune responses were determined by calcein-release assay. Results: V could induce the quickest humoral immune response with the geometrical mean titer of 1: 10(1.6) at week 2 after prime immunization. The antibody titer primed by P ( including PP, PV, PD) mounted up to the highest after the first boost. Antibody induced by PP was more polarized to Th2 while the other groups induced balanced Th1/Th2 response. Among all the groups, VD and DV induced the strongest CTL response. After the fourth boost, the specific lysis ratio was 64 and 71% separately at an E: T ratio of 1:1. Conclusions: P was the most potent for inducing humoral immune response while the weakest for CTL response. D was a poor immunogen to induce specific antibody production. Among all the immune combinations, DV and VD induced the strongest CTL response in Balb/c mice. Copyright (c) 2007 S. Karger AG, Basel.