Bioinformatics analysis of QM-like(PcQM) protein from Pseudosciaena crocea
- 海洋环境－已发表论文 
[目的]通过分析PCQM蛋白质的结构,预测其高级结构和功能,为进一步研究PCQM蛋白提供理论依据。[方法]利用blAST P和相关分析蛋白质理化性质与结构的软件,对PCQM蛋白进行结构与功能预测分析。[结果]PCQM蛋白不存在n端信号肽,存在多个酶切位点,无跨膜结构,定位在细胞质中。不存在二硫键,该蛋白含26.05%α-螺旋结构(H),20.93%β-折叠结构(E),53.02%无规则卷曲(l)。最后,通过SWISS-MOdEl程序预测了PCQM蛋白的三级结构。[结论]较全面地分析和预测了PCQM蛋白的理化性质、二级结构和三级结构等。为深入分析PCQM在大黄鱼(lArIMICHTHyS CrOCEA)体内免疫调节功能研究,提供了理论依据。[Objective]The purpose of this study was to learn more function of PcQM protein by analyzing the AA sequence and predicting the structure of it.[Methods]Some related bioinformatics analysis software or system on internet such as Blast P,Predict Protein,Swiss-Model,was used to analyze the structure and prediction the structure and function of PcQM protein.[Results]The results showed that PcQM protein had many restriction enzyme sites.There was no signal peptide on N-terminal and no transmembrane structure.It was located in the cytoplasm.There were 26.05% α-helical(H),20.93% β-pleated sheet(E) and 53.02% random coil(L) predicted by Predict Protein.The tertiary structure was predicted by Swiss-Model.[Conclusion]The study has analyzed the sequence of PcQM protein and predicted the structure of it,which would lay a foundation for further learning the relationship between structure and function of the protein.