Separation and Identification of β-alanine in Sargassum horneri(Turn.) C.Ag.
- 海洋环境－已发表论文 
非蛋白质氨基酸在抗癌、抗菌、抗结核、抗坏血病等方面有着重要的作用。本文主要对铜藻中的游离氨基酸进行检测分析和部分分离纯化及结构鉴定方面的研究,为更好的开发利用这些天然产物提供技术支持。采用离子交换树脂层析法分离铜藻粗提液中的游离氨基酸,收集3 MOl/l的氨水洗脱液,用PITC-HPlC柱前衍生反相高效液相色谱法对其进行检测分析,结果显示粗提液中除含有多种组成蛋白质的氨基酸外还有3种未知组分,且含量较高的常见蛋白质氨基酸为丙氨酸、脯氨酸、缬氨酸。采用半制备高效液相色谱系统制备分离了其中一种未知组分的衍生物MWz2,经真空冷冻干燥后为略黄固体粉末,结合核磁共振波谱、高分辨质谱、红外光谱数据,最终鉴定MWz2去掉已知取代基团PITC后的成分为β-丙氨酸,分子式为C3H7nO2,分子量为89.09。Non-protein amino acids were found to have many bioactivities such as anti-cancer,antibacterial,anti-tuberculosis,anti-scurvy and so on.Detection,separation and identification of free amino acids in Sargassum horneri(Turn.) C.Ag.were studied in this paper,which provided technical support for development and utilization of these natural products.Free amino acids were separated by ion-exchange column chromatography from the crude extracts of Sargassum horneri(Turn.) C.Ag.Amino acids were eluted with 3 mol/L NH4OH and the eluate was collected.Many protein amino acids and three unknown components were discovered in Sargassum horneri(Turn.) C.Ag.by PITC-HPLC.The main protein amino acids were alanine,proline,valine.One unknown derivative component(MWZ2) was obtained by preparation HPLC.MWZ2 was slightly yellow solid powder after vacuum freeze-drying.According to analysis of NMR,HR-MS,IR spectroscopy data,the molecular structure of MWZ2 had been elucidated.MWZ2 was β-alanine after removed substituent component of PITC,its molecular formula was C3H7NO2,molecular weight was 89.09.