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dc.contributor.authorZhu, Yu-Jing
dc.contributor.authorLiu, Bo
dc.contributor.authorSong, Kang-Kang
dc.contributor.authorLi, Zhi-Cong
dc.contributor.authorPan, Zhi-Zhen
dc.contributor.authorGuo, Yun-Ji
dc.contributor.authorZhou, Jing-Jing
dc.contributor.authorWang, Qin
dc.contributor.authorChen, Qing-Xi
dc.contributor.author陈清西
dc.date.accessioned2011-08-19T12:26:13Z
dc.date.available2011-08-19T12:26:13Z
dc.date.issued2009
dc.identifier.citationJ. Agric. Food Chem., 2009, 57 (12), pp 5518–5523zh_CN
dc.identifier.issn0021-8561
dc.identifier.urihttp://dx.doi.org/doi:10.1021/jf9007554
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/10458
dc.description.abstractTyrosinase (EC 1.14.18.1) is a key enzyme in pigment biosynthesis of organisms. trans-Cinnamaldehyde thiosemicarbazone, a derivative of benzaldehyde thiosemicarbazone, was synthesized as an inhibitor of tyrosinase. The inhibitory effects of this compound on the activity of mushroom tyrosinase were investigated. The results showed that trans-cinnamaldehyde thiosemicarbazone could potently inhibit both monophenolase activity and diphenolase activity of tyrosinase. For monophenolase activity, trans-cinnamaldehyde thiosemicarbazone could not only lengthen the lag time but also decrease the steady-state rate. For diphenolase activity, the IC50 value was determined to be 5.72 mu M. Kinetic analyses showed that trans-cinnamaldehyde thiosemicarbazone was a reversible and mixed type inhibitor on this enzyme. The inhibition constants (K-I and K-IS) were determined to be 4.45 and 8.85 mu M, respectively. Furthermore, the antibacterial activity against Bacillus subtilis, Escherichia coli, Staphyloccocus aureus, Pseudomonas aeruginosa, Klebsiella pneumonia, and Agrobacterium tumefaciens was investigated. The results showed that trans-cinnamaldehyde thiosemicarbazone was more effective against B. subtilis and S. aureus with the same minimum inhibitory concentration (MIC) of 50 mu g/mL and with the same minimum bactericidal concentration (MBC) of 50 mu g/mL.zh_CN
dc.language.isoenzh_CN
dc.publisherAMER CHEMICAL SOCzh_CN
dc.subjectTyrosinasezh_CN
dc.subjecttrans-cinnamaldehyde thiosemicarbazonezh_CN
dc.subjectantityrosinase activityzh_CN
dc.subjectinhibition kineticszh_CN
dc.subjectantimicrobial activityzh_CN
dc.titleAntityrosinase and Antimicrobial Activities of trans-Cinnamaldehyde Thiosemicarbazonezh_CN
dc.typeArticlezh_CN


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