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dc.contributor.authorTian, Y.
dc.contributor.authorLuo, Y. R.
dc.contributor.authorHong, H. S.
dc.contributor.authorZheng, T. L.
dc.contributor.author郑天凌
dc.date.accessioned2011-08-16T13:08:03Z
dc.date.available2011-08-16T13:08:03Z
dc.date.issued2009
dc.identifier.citationMarine Pollution Bulletin,Volume 58, Issue 8, August 2009, Pages 1159-1163zh_CN
dc.identifier.issn0025-326X
dc.identifier.urihttp://dx.doi.org/doi:10.1016/j.marpolbul.2009.03.024
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/10434
dc.description.abstractA microbial consortium was obtained by enrichment culture of sea water samples collected from Botan oil port in Xiamen, China, using the persistent high concentration of a mixture of polycyclic aromatic hydrocarbons enrichment strategy. Denaturing gradient gel electrophoresis (DGGE) was used to investigate the bacterial composition and community dynamic changes based on PCR amplification of 16S rRNA genes during batch culture enrichment. Using the spray-plate method, three bacteria, designated as BL01, BL02 and BL03, which corresponded to the dominant bands in the DGGE profiles, were isolated from the consortium. Sequence analysis showed that BL01, BL02 and BL03 were phylogenetically close to Ochrobactrum sp., Stenotrophomonas maltophilia and Pseudomonas fluorescens, respectively. The degradation of benzo(a)pyrene (BaP), a model high-molecular-weight polycyclic aromatic hydrocarbon (HMW PAH) compound was investigated using individual isolates, a mixture of the three isolates, and the microbial consortium (BL) originally isolated from the oil port sea water. Results showed that the order of degradative ability was BL > the mixture of the three isolates > individual isolates. BL degraded 44.07% of the 10 ppm BaP after 14 days incubation, which showed the highest capability for HMW PAH compound degradation. Our results revealed that this high selective pressure strategy was feasible and effective in enriching the HMW PAH-degraders from the original sea water samples. (C) 2009 Elsevier Ltd. All rights reserved.zh_CN
dc.description.sponsorshipThe National Natural Science Foundation of China [40876061, 30530150]; Program forChangjiang Scholars and Innovative Research Team in University [40521003]; Science and Technology Foundation of Fujian Province [2008Y0061]; National Infrastructure of Natural Resources for Science and Technology Program of China [2005DKA21209]; Key Laboratory for Ecological Environment in Coastal Areas ; State Oceanic Administration [200703]; State Key Laboratory of Marine Environmental Science (Xiamen University) [MEL0702]zh_CN
dc.language.isoenzh_CN
dc.publisherPERGAMON-ELSEVIER SCIENCE LTDzh_CN
dc.subjectBenzo(a)pyrene (BaP)zh_CN
dc.subjectBiodegradationzh_CN
dc.subjectCommunity structurezh_CN
dc.subjectDenaturing gradient gel electrophoresis (DGGE)zh_CN
dc.subjectHigh selective pressurezh_CN
dc.titleAnalysis of community structure of a microbial consortium capable of degrading benzo(a)pyrene by DGGEzh_CN
dc.typeArticlezh_CN


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