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dc.contributor.authorLi, Guang
dc.contributor.authorZhang, Qiu-Jin
dc.contributor.authorZhong, Jing
dc.contributor.authorWang, Yi-Quan
dc.contributor.author王义全
dc.date.accessioned2011-08-16T12:54:16Z
dc.date.available2011-08-16T12:54:16Z
dc.date.issued2009
dc.identifier.citationGene,Volume 446, Issue 1, 1 October 2009, Pages 41-49zh_CN
dc.identifier.issn0378-1119
dc.identifier.urihttp://dx.doi.org/doi:10.1016/j.gene.2009.07.003
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/10432
dc.description.abstractGreen fluorescent protein (GFP) has been widely used as a molecular marker in modern biological research. Before the recent report of one GFP gene in Branchiostoma floridae, GFP family members were cloned only from other two groups of species: Cnidaria and Copepoda. Here we describe the complete GFP gene repertoire of B. floridae which includes 13 functional genes and 2 pseudogenes, representing the largest GFP family found so far. Coupling with nine other GFP sequences from another two species of genus Branchiostoma and the sequences from Cnidaria and Copepoda, we made a deep-level phylogenetic analysis for GFP genes in cephalochordates and found: 1) GFP genes have experienced a divergent evolution in cephalochordates; 2) all amphioxus GFP genes form four main clades on the tree which had diverged before the radiation of the last common ancestor of all extant cephalochordates; 3) GFP genes in amphioxus shared a common ancestor with that in Copepoda rather than being derived from horizontal gene transfer, which indicates that our ancestor was derived from a fluorescent organism and lost this ability after its separation from Cephalochordata, and also makes GFP a rare gene which has a rather unusual evolutionary path. In addition, we also provided evidence indicating that GFP genes have evolved divergent functions by specializing their expression profile, and different fluorescent spectra by changing their emission peaks. These findings spark two interesting issues: what are GFP in vivo functions in cephalochordates and why they are lost in other examined deuterostomes? (C) 2009 Elsevier B.V. All rights reserved.zh_CN
dc.description.sponsorshipNational Natural Science Foundation of China [30830023]; National High Technology Research and Development Program of China (863 Program) [2008AA092602]zh_CN
dc.language.isoenzh_CN
dc.publisherELSEVIER SCIENCE BVzh_CN
dc.subjectGFPzh_CN
dc.subjectAmphioxuszh_CN
dc.subjectCephalochordatezh_CN
dc.subjectEvolutionzh_CN
dc.titleEvolutionary and functional diversity of green fluorescent proteins in cephalochordateszh_CN
dc.typeArticlezh_CN


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