• 中文
    • English
  • English 
    • 中文
    • English
  • Login
View Item 
  •   DSpace Home
  • 生命科学学院
  • 生命科学-已发表论文
  • View Item
  •   DSpace Home
  • 生命科学学院
  • 生命科学-已发表论文
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Evolutionary and functional diversity of green fluorescent proteins in cephalochordates

Thumbnail
Full Text
Evolutionary and functional diversity of green fluorescent proteins in cephalochordates.htm (413bytes)
Date
2009
Author
Li, Guang
Zhang, Qiu-Jin
Zhong, Jing
Wang, Yi-Quan
王义全
Collections
  • 生命科学-已发表论文 [5876]
Show full item record
Abstract
Green fluorescent protein (GFP) has been widely used as a molecular marker in modern biological research. Before the recent report of one GFP gene in Branchiostoma floridae, GFP family members were cloned only from other two groups of species: Cnidaria and Copepoda. Here we describe the complete GFP gene repertoire of B. floridae which includes 13 functional genes and 2 pseudogenes, representing the largest GFP family found so far. Coupling with nine other GFP sequences from another two species of genus Branchiostoma and the sequences from Cnidaria and Copepoda, we made a deep-level phylogenetic analysis for GFP genes in cephalochordates and found: 1) GFP genes have experienced a divergent evolution in cephalochordates; 2) all amphioxus GFP genes form four main clades on the tree which had diverged before the radiation of the last common ancestor of all extant cephalochordates; 3) GFP genes in amphioxus shared a common ancestor with that in Copepoda rather than being derived from horizontal gene transfer, which indicates that our ancestor was derived from a fluorescent organism and lost this ability after its separation from Cephalochordata, and also makes GFP a rare gene which has a rather unusual evolutionary path. In addition, we also provided evidence indicating that GFP genes have evolved divergent functions by specializing their expression profile, and different fluorescent spectra by changing their emission peaks. These findings spark two interesting issues: what are GFP in vivo functions in cephalochordates and why they are lost in other examined deuterostomes? (C) 2009 Elsevier B.V. All rights reserved.
Citation
Gene,Volume 446, Issue 1, 1 October 2009, Pages 41-49
URI
http://dx.doi.org/doi:10.1016/j.gene.2009.07.003
https://dspace.xmu.edu.cn/handle/2288/10432

copyright © 2002-2016  Duraspace  Theme by @mire  厦门大学图书馆  
About | Policies
 

 

Browse

All of DSpaceCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

My Account

LoginRegister

copyright © 2002-2016  Duraspace  Theme by @mire  厦门大学图书馆  
About | Policies