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dc.contributor.authorZhang, Guo-guan
dc.contributor.authorZhang, Hui-huang
dc.contributor.authorLi, Dong-xiao
dc.contributor.authorZeng, Ya-ming
dc.contributor.authorChen, Liang
dc.contributor.author陈亮
dc.date.accessioned2011-08-15T12:59:13Z
dc.date.available2011-08-15T12:59:13Z
dc.date.issued2009
dc.identifier.citationVeterinary Research Communications,Volume 33, Number 7, 735-747zh_CN
dc.identifier.issn0165-7380
dc.identifier.urihttp://dx.doi.org/doi:10.1007/s11259-009-9222-7
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/10419
dc.description.abstractM2e is the external domain of M2 protein, a conservative transmembrane protein of the avian influenza A virus. Previous research had shown that the vaccine of the formation particle of M2e and hepatitis B virus core antigen (HBcAg) can fully protect mice against a lethal H5N1 subtype avian influenza virus (AIV) infection. As an effective approach against mucosal tissue infectious agent, mucosal vaccination requires effective and safe adjuvants. Here we have first fused two M2e peptide to the N terminal and the major immunodominant region (MIR) of the HBcAg protein simultaneously to create a fusion gene, named as M2eHBc+, and then inserted B subunit of Escherichia coli heat labile enterotoxin (LTB) into the N terminal of M2eHBc+ to construct the second fusion gene, named as LBM2eHBc+. These two fusion genes can be efficiently expressed in Escherichia coli cell and the yield peptide can self-assemble into virus-like particles (VLP). The mice immunization with two types of the purified particles by intranasal dropping and oral routes revealed that LTB can significantly enhance the mucosal immune responses of mice to co-expression M2eHBc+ particle form antigen.zh_CN
dc.description.sponsorshipimportant special project fund of Fujian provincezh_CN
dc.language.isoenzh_CN
dc.publisherSPRINGERzh_CN
dc.subjectAvian influenza viruszh_CN
dc.subjectHBcAgzh_CN
dc.subjectM2eHBc+zh_CN
dc.subjectLTBzh_CN
dc.subjectMucosal immunityzh_CN
dc.titleEnhancement of mucosal immune response against the M2eHBc+antigen in mice with the fusion expression products of LTB and M2eHBc+through mucosal immunization routezh_CN
dc.typeArticlezh_CN


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