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dc.contributor.authorLi, Qi-Fu
dc.contributor.author李祺福
dc.contributor.authorShi, Song-Lin
dc.contributor.authorSong, Jianye
dc.contributor.authorTang, Jian
dc.contributor.authorLiang, Ying
dc.contributor.authorLiu, Qing-Rong(NIDA IRP)
dc.date.accessioned2011-08-12T09:37:57Z
dc.date.available2011-08-12T09:37:57Z
dc.date.issued2008
dc.identifier.citationThe International Journal of Biochemistry & Cell Biology,Volume 40, Issue 9, 2008, Pages 1918-1929zh_CN
dc.identifier.issn1357-2725
dc.identifier.urihttp://dx.doi.org/doi:10.1016/j.biocel.2008.01.031
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/10389
dc.description.abstractGinsenoside RgI, cinnamic acid, and tanshinone IIA are effective anticancer and antioxidant constituents of traditional Chinese herbal medicines of Ginseng (Panax ginseng), Xuanshen (Radix scrophulariae), and Danshen (Salvia mitiorrhiza), respectively. There was insufficient study on molecular mechanisms of anticancer effects of those constituents and their targets were unknown. We chose nucleophosmin as a candidate molecular target because it is frequently mutated and upregulated in various cancer cells. Nucleophosmin is a major nucleolus phosphoprotein that involves in rRNA synthesis, maintaining genomic stability, and normal cell division and its haploinsufficiency makes cell more susceptible to oncogenic assault. Ginsenoside RgI, cinnarnic acid, and tanshinone IIA treatment of osteosarcoma MG-63 cells decreased nucleophosmin expression in nuclear matrix and induced nucleophosmin translocation from nucleolus to nucleoplasm and cytoplasm, a process of dedifferentiating transformed cells. Using immunogold electro-microscopy, we found at the first time that nucleophosmin was localized on nuclear matrix intermediate filaments that had undergone restorational changes after the treatments. Nucleophosmin also functions as a molecular chaperone that might interact with multiple oncogenes and tumor suppressor genes. We found that oncogenes c-myc, c-fos and tumor suppressor genes, P53, Rb were regulated by ginsenoside Rg I, cinnamic acid, and tanshinone IIA as well. In present study, we identified nucleophosmin as a molecular target of the effecfive anticancer constituents of t Ginseng, Xuanseng, and Danseng that down-regulated nucleophosmin in nuclear matrix, changed its trafficking from nucleolus to cytoplasm, and regulated several oncogenes and tumor suppressor genes. Therefore, we postulate that Ginsenoside Rgl, cinnamic acid, and tanshinone HA could serve as protective agents in cancer prevention and treatment. (c) 2008 Elsevier Ltd. All rights reserved.zh_CN
dc.language.isoenzh_CN
dc.publisherPERGAMON-ELSEVIER SCIENCE LTDzh_CN
dc.subjectginsenosidezh_CN
dc.subjectnucleophosminzh_CN
dc.subjecthuman osteosarcomazh_CN
dc.subjectnuclear matrixzh_CN
dc.subjectinduced differentiationzh_CN
dc.titleAnticancer effects of ginsenoside Rg1, cinnamic acid, and tanshinone IIA in osteosarcoma MG-63 cells: Nuclear matrix downregulation and cytoplasmic trafficking of nucleophosminzh_CN
dc.typeArticlezh_CN


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