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dc.contributor.authorHu, Shi-Jun
dc.contributor.authorYang, Zeng-Ming
dc.contributor.author杨增明
dc.contributor.authorRen, Gang(NE Agr Univ)
dc.contributor.authorNi, Hua(NE Agr Univ)
dc.contributor.authorLiu, Ji-Long(NE Agr Univ)
dc.contributor.authorZhao, Zhen-Ao(NE Agr Univ)
dc.contributor.authorYu, Yong-Sheng(NE Agr Univ)
dc.contributor.authorSu, Ren-Wei(NE Agr Univ)
dc.contributor.authorMa, Xing-Hong(NE Agr Univ)
dc.contributor.authorLei, Wei(NE Agr Univ)
dc.date.accessioned2011-08-12T09:12:35Z
dc.date.available2011-08-12T09:12:35Z
dc.date.issued2008
dc.identifier.citationJOURNAL OF BIOLOGICAL CHEMISTRY,283, 23473-23484.zh_CN
dc.identifier.issn0021-9258
dc.identifier.urihttp://dx.doi.org/doi:10.1074/jbc.M800406200
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/10386
dc.description.abstractMicroRNAs (miRNAs) are 21-24-nucleotide non-coding RNAs found in diverse organisms. Although hundreds of miRNAs have been cloned or predicted, only very few miRNAs have been functionally characterized. Embryo implantation is a crucial step in mammalian reproduction. Many genes have been shown to be significantly changed in mouse uterus during embryo implantation. However, miRNA expression profiles in the mouse uterus between implantation sites and inter-implantation sites are still unknown. In this study, miRNA microarray was used to examine differential expression of miRNAs in the mouse uterus between implantation sites and inter-implantation sites. Compared with inter-implantation sites, there were 8 up-regulated miRNAs at implantation sites, which were confirmed by both Northern blot and in situ hybridization. miR-21 was highly expressed in the subluminal stromal cells at implantation sites on day 5 of pregnancy. Because miR-21 was not detected in mouse uterus during pseudopregnancy and under delayed implantation, miR-21 expression at implantation sites was regulated by active blastocysts. Furthermore, we showed that Reck was the target gene of miR-21. Our data suggest that miR-21 may play a key role during embryo implantation.zh_CN
dc.description.sponsorshipNational Basic Research Program of China [2006CB504005, 2006CB944006]; Chinese National Natural Science Foundation [30570198, 30770244]zh_CN
dc.language.isoenzh_CN
dc.publisherAMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INCzh_CN
dc.titleMicroRNA expression and regulation in mouse uterus during embryo implantationzh_CN
dc.typeArticlezh_CN


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