Novosphingobium indicum sp nov., a polycyclic aromatic hydrocarbon-degrading bacterium isolated from a deep-sea environment
- 生命科学－已发表论文 
A novel polycyclic aromatic hydrocarbon (PAH)-degrading bacterium, strain H25(T), which was isolated from deep-sea water of the Indian Ocean, was studied phenotypically, genotypically and phylogenetically. Strain H25(T) can utilize several PAHs including phenanthrene and fluoranthene as sole carbon sources. The 16S rRNA gene sequence of strain H25(T) showed the highest similarity with that of Novosphingobium naphthalenivorans TUT562(T) (96.3%), and showed lower similarities (92.1-96.0%) with other members of the genus Novosphingobium. The major fatty acids of strain H25(T) were C-14:0 2-OH (3.2%), C-16:0 (13.6%), C-16:1 omega 7c (5.2%), C-18:0 (113.4%) and C18-1 omega 7c (57.0%), which accounted for 92.3% of the total fatty acids. It had ubiquinone 10 as the major respiratory quinone and spermidine as the major polyamine. All these characteristics were consistent with those of recognized Novosphingobium species. Results of DNA-DNA hybridization experiments and BOX-PCR fingerprint comparisons also indicate that strain H25(T) represents a novel Novosphingobium species, for which the name Novosphingobium indicum sp. nov. is proposed. The type strain is H25(T) (=MCCC 1A01080(T)=CGMCC 1.6784(T)=LMG 24713(T)).