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dc.contributor.authorLee, Kai-Fai ( Univ Hong Kong)
dc.contributor.authorTam, Yuen-Tsung ( Univ Hong Kong)
dc.contributor.authorZuo, Yan ( Univ Hong Kong)
dc.contributor.authorCheong, Ana W. Y. ( Univ Hong Kong)
dc.contributor.authorPang, Ronald T. K. ( Univ Hong Kong)
dc.contributor.authorYeung, William S. B. ( Univ Hong Kong)
dc.contributor.authorLee, Nikki P. Y. ( Univ Hong Kong)
dc.contributor.authorLuk, John M. C. ( Univ Hong Kong)
dc.contributor.authorShum, Cathy K. Y. ( Univ Hong Kong)
dc.contributor.authorTam, Po-Chor ( Univ Hong Kong)
dc.contributor.authorCheung, Annie N. Y. ( Univ Hong Kong)
dc.contributor.authorYang, Zeng-Ming
dc.contributor.author杨增明
dc.date.accessioned2011-07-31T07:20:26Z
dc.date.available2011-07-31T07:20:26Z
dc.date.issued2008
dc.identifier.citationMol. Hum. Reprod. (2008) 14 (8): 465-474.zh_CN
dc.identifier.issn1360-9947
dc.identifier.urihttp://dx.doi.org/doi:10.1093/molehr/gan041
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/10345
dc.description.abstractThe release of enzymes from the acrosome of the sperm head (acrosome reaction) starts the fertilization process and enables the spermatozoa to penetrate the zona pellucida of the oocytes. Defective acrosome reaction is one of the important causes of infertility in men. To investigate the molecular regulation of spermatogenesis in vivo, we used differential display reverse transcription polymerase chain reaction to identify stage-specific genes in a retinol-supplemented vitamin-A deficiency (VAD) rat model and identified the VAD1.2 (acrosome-expressed protein 2, AEP2) gene, which was expressed strongly in the rat testis from postnatal day 32 to adult stage. The mouse VAD1.2 mRNA shared 85% and 67% sequence homology, and 74% and 38% amino acid homology, respectively, with the rat and human counterparts. VAD1.2 transcript was abundantly expressed in the rat seminiferous tubules at stage VIII-XII, and the protein was detected in the acrosome region of the round and elongated spermatids of mouse, human, monkey and pig. VAD1.2 co-localized with lectin-PNA to the acrosome region of spermatids. Interestingly, the expression of VAD1.2 protein in human testis diminished in patients with hypospermatogenesis, maturation arrest, undescended testis and Sertoli cell-only syndrome. Co-immunoprecipitation experiments followed by western blotting and mass spectrometry (MS-MS) identified syntaxin 1, beta-actin and myosin heavy chain (MHC) proteins as putative interacting partners. Taken together, the stage-specific expression of VAD1.2 in the acrosome of spermatids and the binding of VAD1.2 protein with vesicle forming (syntaxin 1) and structural (beta-actin and MHC) proteins suggest that VAD1.2 maybe involved in acrosome formation during spermiogenesis.zh_CN
dc.description.sponsorshipThe University of Hong Kong's Committee on Research and Conference ; Hong Kong Research Grant Council [HKU7272/01M, HKU7537/05M, N_HKU712/06]zh_CN
dc.language.isoenzh_CN
dc.publisherOXFORD UNIV PRESSzh_CN
dc.subjectspermatogenesiszh_CN
dc.subjectacrosomezh_CN
dc.subjectgolgi apparatuszh_CN
dc.subjectsyntaxinzh_CN
dc.subjectbeta-actinzh_CN
dc.titleCharacterization of an acrosome protein VAD1.2/AEP2 which is differentially expressed in spermatogenesiszh_CN
dc.typeArticlezh_CN


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