• 中文
    • English
  • English 
    • 中文
    • English
  • Login
View Item 
  •   DSpace Home
  • 生命科学学院
  • 生命科学-已发表论文
  • View Item
  •   DSpace Home
  • 生命科学学院
  • 生命科学-已发表论文
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Trapping Capacities, Stability and Interaction Intensity of Subunits from Bacterial Ferritin of Azotobacter Vinelandii

Thumbnail
Full Text
Trapping Capacities, Stability and Interaction Intensity of Subunits from Bacterial Ferritin of Azotobacter Vinelandii.htm (413bytes)
Date
2008-08
Author
Huang Lin
Chen Xu
Luo Lian-Zhong
Lin Qing
Huang He-Qing
黄河清
Collections
  • 生命科学-已发表论文 [5901]
Show full item record
Abstract
Bacterial ferritin with purity for the mass spectrometric analysis was prepared by the column chromatography, the electrophoresis and the RP-HPLC in Azotobacter vinelandii. Bacterial ferritin of Azotobacter vinelandii (AVBF) was further identified by both kinetics of iron release and peptide mass fingerprinting (PMF), respectively. Moreover, the interaction Intensity, stability and polymer among subunits in AVBF were further revealed by both MALDI time of flight mass spectrometry and electrophoresis. AVBF can be used to trap organic small molecules such as methylene blue (MB) and the trapping rate is approximately 15.0 +/- 2.0 MB/AVBF, which mdicates that the heme component located at the interface between monomers of the ferritn subunit participates contributes to trap MB. AVBF and liver ferritin of shark (SLF) can directly release its unstable subunits for the mass spectrometric analysis under the 40%-50% condition both acetonitrile and acetone, respectively, but both proteins can not release the subunits for the analysis under the condition of 20%-30% acetone unless both proteins absorb the laser from the mass spectrometer further. It was well known that the interaction intensity among subunits in AVBF was lower than that of SLF. The interaction intensity among protein subunits were tightly connected with the rate of iron release and storage in ferritin.
Citation
Chinese Journal of Analytical Chemistry,Volume 36, Issue 8, August 2008, Pages 1045-1050
URI
http://dx.doi.org/doi:10.1016/S1872-2040(08)60057-2
https://dspace.xmu.edu.cn/handle/2288/10338

copyright © 2002-2016  Duraspace  Theme by @mire  厦门大学图书馆  
About | Policies
 

 

Browse

All of DSpaceCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

My Account

LoginRegister

copyright © 2002-2016  Duraspace  Theme by @mire  厦门大学图书馆  
About | Policies