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dc.contributor.authorLin Zhi-cao
dc.contributor.authorHuang Lin
dc.contributor.authorLin Qing
dc.contributor.authorLuo Lian-zhong
dc.contributor.authorHuang He-qing
dc.contributor.author黄河清
dc.date.accessioned2011-07-31T02:48:58Z
dc.date.available2011-07-31T02:48:58Z
dc.date.issued2008
dc.identifier.citationChemical Research in Chinese Universities,Volume 24, Issue 5, September 2008, Pages 550-556zh_CN
dc.identifier.issn1005-9040
dc.identifier.urihttp://dx.doi.org/doi:10.1016/S1005-9040(08)60116-6
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/10330
dc.description.abstractPig pancreas ferritin(PPF) was purified by ultra-centrifugation, ion-exchange chromatography, and native gradient polyacrylamide gel electrophoresis(PAGE(NG)). Sodium dodecyl sulfate(SDS)-PAGE indicates that PPF consists of two subunit types, namely, H(21000) and L(19000) subunits, and its core shows an average element composition of 1698 Fe3+ and 179 phosphate molecules within the hollow shell, giving a 9.51 ratio of Fe3+ to phosphate. An off line approach combining reversed-phase high-performance liquid chromatography(RP-HPLC) with matrix-assisted laser desorption ionization time of flight mass spectrometry(MALDI-TOF MS) made the decomposition of PPF shell into H and L subunits for the analysis of mass spectrometry(MS), giving molecular weights of both H(21014.4) and L(18319.9) subunits. Both subunit types were further identified by an approach combining peptide mass fingerprint(PMF) with database search. A ratio of 1H to 2L subunits in PPF was determined by SDS-PAGE, RP-HPLC, and MALDI-TOF MS. respectively. It is well known that the non-covalent interaction of L-L or H-L subunits is stronger than that of H-H subunits in PPF, which may be further used to explain the unclear physiological function between H and L subunits in PPF.zh_CN
dc.description.sponsorshipNational Natural Science Foundation of China [40776060]; Program for Innovative Research Team in Science and Technology in Fujian Province Universityzh_CN
dc.language.isoenzh_CN
dc.publisherHIGHER EDUCATION PRESSzh_CN
dc.subjectFerritinzh_CN
dc.subjectPig pancreaszh_CN
dc.subjectSubunit structurezh_CN
dc.subjectProteomiczh_CN
dc.subjectMALDI-TOF MSzh_CN
dc.subjectRP-HPLCzh_CN
dc.titleSubunit Characteristics of Pig Pancreas Ferritin Revealed by MALDI-TOF MS and RP-HPLCzh_CN
dc.typeArticlezh_CN


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