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dc.contributor.authorPan, Jian-yi
dc.contributor.authorWang, San-ying
dc.contributor.author王三英
dc.contributor.authorPeng, Xuan-xian
dc.contributor.author彭宣宪
dc.contributor.authorZhang, Yue-ling(Shantou Univ)
dc.date.accessioned2011-07-31T02:29:16Z
dc.date.available2011-07-31T02:29:16Z
dc.date.issued2008
dc.identifier.citationMarine Biotechnology ,Volume 10, Number 6, 645-652zh_CN
dc.identifier.issn1436-2228
dc.identifier.urihttp://dx.doi.org/doi:10.1007/s10126-008-9115-8
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/10328
dc.description.abstractHemocyanins are multi-functional proteins, although they are well known to be respiratory proteins of invertebrate to date. In the present study, the agglutination ability of two oligomers of hemocyanin, hexamer and dodecamer, with pathogenic bacteria and red blood cells (RBCs) is investigated in pacific white shrimp, Litopenaeus vannamei. Hexameric hemocyanin exhibits an extremely high stability even in the absence of Ca2+ and in alkaline pH. Dodecamer (di-hexamer) is easily dissociated into hexamers in unphysiological conditions. Hexamer and dodecamer are interchanged reciprocally with environmental conditions. Both oligomers can bind to bacteria and RBCs, but agglutination is observed only using dodecamer but not using hexamer in agglutination assay. However, the agglutination is detected when hexamer is utilized in the presence of antiserum against hemocyanin. These results indicate that dodecamer of hemocyanin is required for agglutination with bacteria and RBCs. It can be logically inferred that there is only one carbohydrate-binding site to bacterial cells and RBCs in the hexamer, while at least two sites in the dodecamer. Our finding has provided new insights into structural-functional relationship of hemocyanin.zh_CN
dc.description.sponsorshipNational Basic Research Program of China [2006CB101807]; Guangdong Natural Science Foundation [7117645]; Science and Technology Program of Guangdong [2007B020701006]; Shantou City [2006-148]zh_CN
dc.language.isoenzh_CN
dc.publisherSPRINGERzh_CN
dc.subjectHemocyaninzh_CN
dc.subjectAgglutinationzh_CN
dc.subjectHexamerzh_CN
dc.subjectDodecamerzh_CN
dc.subjectLitopenaeus vannameizh_CN
dc.titleDodecamer is Required for Agglutination of Litopenaeus vannamei Hemocyanin with Bacterial Cells and Red Blood Cellszh_CN
dc.typeArticlezh_CN


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