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dc.contributor.authorLi, Qingge
dc.contributor.author李庆阁
dc.contributor.authorXia, Xiaohu
dc.contributor.authorXu, Ye
dc.contributor.author许晔
dc.contributor.authorZhao, Xilin
dc.date.accessioned2011-07-30T08:11:55Z
dc.date.available2011-07-30T08:11:55Z
dc.date.issued2009
dc.identifier.citationClinical Chemistry 55: 179-182, 2009zh_CN
dc.identifier.issn0009-9147
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/10311
dc.description.abstractBACKGROUND: Despite their ease of use, lateral flow immunoassays (LFIAs) often suffer from poor quantitative discrimination and low analytical sensitivity. We explored the use of a novel class of europium chelate-loaded silica nanoparticles as labels to overcome these limitations. METHODS: Antibodies were covalently conjugated onto europium chelate-loaded silica nanoparticles with dextran as a linker. The resulting conjugates were used as labels in LFIA for detection of hepatitis B surface antigen (HBsAg). We performed quantification with a digital camera and Adobe Photoshop software. We also used 286 clinical samples to compare the proposed method with a quantitative ELISA. RESULTS: A detection limit of 0.03 mu g/L was achieved, which was 100 times lower than the colloidal gold-based LFIAs and lower than ELISA. A precise quantitative dose-response curve was obtained, and the linear measurement range was 0.05-3.13 mu g/L, within which the CVs were 2.3%-10.4%. Regression analysis of LFIA on ELISA results gave: log (LFIA) = -0.14 log (ELISA) + 1.03 mu g/L with r = 0.99 for the quantification of HBsAg in 35 positive serum samples. Complete agreement was observed for the qualitative comparison of 286 clinical samples assayed with LFIA and ELISA. CONCLUSIONS: Europium chelate-loaded silica nano-particle labels have great potential to improve LFIAs, making them useful not only for simple screening applications but also for more sensitive and quantitative immunoassays.zh_CN
dc.description.sponsorshipXiamen Scientific Development Program [3502Z20055008]zh_CN
dc.language.isoenzh_CN
dc.publisherAMER ASSOC CLINICAL CHEMISTRYzh_CN
dc.titleLateral Flow Immunoassay Using Europium Chelate-Loaded Silica Nanoparticles as Labelszh_CN
dc.typeArticlezh_CN


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