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dc.contributor.authorLing, Xue-Ping
dc.contributor.authorZhu, Jin-Yong
dc.contributor.authorHuang, Lin
dc.contributor.authorHuang, He-Qing
dc.contributor.author黄河清
dc.date.accessioned2011-07-30T02:31:53Z
dc.date.available2011-07-30T02:31:53Z
dc.date.issued2009
dc.identifier.citationEnvironmental Toxicology and Pharmacology,Volume 27, Issue 2, March 2009, Pages 212-218zh_CN
dc.identifier.issn1382-6689
dc.identifier.urihttp://dx.doi.org/doi:10.1016/j.etap.2008.10.004
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/10291
dc.description.abstractin the present study, we developed a two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) technique for examining the response of the proteome from gill tissue of Paralichthys olivaceus (POGT) to acute cadmium (AC) toxicity. Approximately 700 protein spots were detected from the gill sample when applying a 600 mu g protein 2D-PAGE gel in the pH range 5.0-8.0, and approximately 400 of these were identified by peptide mass fingerprinting (PMF) and database search. Compared to a control sample, significant changes were visualized in 18 protein spots exposed to seawater cadmium acute toxicity at 10.0 ppm for 24 h. Among these spots, two were up-regulated, one was down-regulated, seven showed low expression, and eight showed high expression. The collected spots were further identified by PMF and database search. Ten of the 18 proteins identified on the 2D-PAGE gel, including heat shock protein 70 and calcium-binding protein, demonstrated a synchronous response to AC, and we suggest that the variable levels and trends of these spots on the gel might be utilized as biomarker profiles to investigate cadmium contamination levels in seawater and to evaluate the degree of risk of human fatalities. The experimental results emphasize that the application of multiple biomarkers has an advantage over single biomarkers for monitoring levels of heavy metal contamination in seawater. (C) 2008 Elsevier B.V. All rights reserved.zh_CN
dc.description.sponsorshipState Natural Science Fund [40776060]; Xiamen University [2004xdcx 207, xdkjcx 200510009, 2005-113]zh_CN
dc.language.isoenzh_CN
dc.publisherELSEVIER SCIENCE BVzh_CN
dc.subjectProteomicszh_CN
dc.subjectAcute cadmiumzh_CN
dc.subjectSignificant changezh_CN
dc.subjectGill tissuezh_CN
dc.subjectInduced proteinszh_CN
dc.subjectParalichthys olivaceuszh_CN
dc.titleProteomic changes in response to acute cadmium toxicity in gill tissue of Paralichthys olivaceuszh_CN
dc.typeArticlezh_CN


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