• 中文
    • English
  • English 
    • 中文
    • English
  • Login
View Item 
  •   DSpace Home
  • 化学化工学院
  • 化学化工-已发表论文
  • View Item
  •   DSpace Home
  • 化学化工学院
  • 化学化工-已发表论文
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Optimized separation and identification of proteome from the baccal ganalion of Aplysia(Notarcus leachii cirrosus Stimpson)

Thumbnail
Full Text
优化分离与鉴定蓝斑背肛海兔口腔神经节蛋白质组.pdf (3.065Mb)
Date
2006
Author
Chen Dong-Shi
陈东仕
Huang He-Qing
黄河清
Wu Han-Zhi
吴韩志
Cai Zong-Wei
蔡宗苇
Collections
  • 化学化工-已发表论文 [14469]
Show full item record
Abstract
A separation method of proteome from the buccal ganglion, of aplysia (Notarcus leachii cirrosus Stimpson, NLCS) with two-dimensional polyacrylamide gel electrophoresis(2D-PAGE) was optimized, gaining about 300 protein spots. Mass spectrogram of peptide mass fingerprint(PMF) of 96 protein spots from the BG were obtained by a combined off-line technique of the matrix-assisted laser desorption ionization mass spectrometry(MALDI-TOF MS) and the emzymolysis in gel. Based on the identification via the databases searches and PMF maps, we found that four of 96 proteins in the gel showed the characteristics with high match scores, which were indicated to be framework proteins tubulin, actin, ribulose-1, 5-bisphosphate carboxylase and oxygenase in neurons cell; meanwhile, a peptide mating pheromone precursor was found. Fifty-six proteins in BG were further classified by a LOC tree software according to the search result of subcellular localization.
Citation
CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,2006,27(7):1257-1261
URI
https://dspace.xmu.edu.cn/handle/2288/10283

copyright © 2002-2016  Duraspace  Theme by @mire  厦门大学图书馆  
About | Policies
 

 

Browse

All of DSpaceCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

My Account

LoginRegister

copyright © 2002-2016  Duraspace  Theme by @mire  厦门大学图书馆  
About | Policies