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dc.contributor.authorSun, Yi
dc.contributor.authorZhang, Qiu-Jin
dc.contributor.authorZhong, Jing
dc.contributor.authorWang, Yi-Quan
dc.contributor.author王义权
dc.date.accessioned2011-07-23T06:57:44Z
dc.date.available2011-07-23T06:57:44Z
dc.date.issued2010-02
dc.identifier.citationDevelopment, Growth & Differentiation,volum52,issue2,pp157-167zh_CN
dc.identifier.issn0012-1592
dc.identifier.urihttp://dx.doi.org/doi:10.1111/j.1440-169X.2009.01150.
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/10273
dc.description.abstractBone morphogenetic proteins (BMPs) are responsible for regulating embryo development and tissue homeostasis beyond osteogenesis. However, the precise biological roles of BMP3 and BMP3b remain obscure to a certain extent. In the present study, we cloned an orthologous gene (AmphiBMP3/3b) from amphioxus (Branchiostoma japonicum) and found its exon/intron organization is highly conserved. Further, in situ hybridization revealed that the gene was strongly expressed in the dorsal neural plate of the embryos. The gene also appeared in Hatschek's left diverticulum, neural tube, preoral ciliated pit and gill slit of larvae, and adult tissues including ovary, neural tube and notochordal sheath. Additionally, real-time quantitative polymerase chain reaction (RTqPCR) analysis revealed that the expression displayed two peaks at gastrula and juvenile stages. These results indicated that AmphiBMP3/3b, a sole orthologue of vertebrate BMP3 and BMP3b, might antagonize ventralizing BMP2 orthologous signaling in embryonic development, play a role in the evolutionary precursors of adenohypophysis, as well as act in female ovary physiology in adult.zh_CN
dc.description.sponsorshipNational Natural Science Foundation of China [30830023]; National High Technology Research and Development Program of China (863 Program) [2008AA092602]; Ministry of Education, China [20070384041]zh_CN
dc.language.isoenzh_CN
dc.publisherWILEY-BLACKWELL PUBLISHING, INCzh_CN
dc.subjectamphioxuszh_CN
dc.subjectBMP3zh_CN
dc.subject3b genezh_CN
dc.subjectdevelopmental embryozh_CN
dc.subjectevolutionzh_CN
dc.subjectgene expression patternzh_CN
dc.titleCharacterization and expression of AmphiBMP3/3b gene in amphioxus Branchiostoma japonicumzh_CN
dc.typeArticlezh_CN


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