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dc.contributor.authorYe, Zuyun
dc.contributor.authorTian, Hui Qiao
dc.contributor.author田惠桥
dc.contributor.authorWang, Ya Ying
dc.date.accessioned2011-07-23T02:32:37Z
dc.date.available2011-07-23T02:32:37Z
dc.date.issued2010
dc.identifier.citationActa Biologica Cracoviensia. Series Botanica 2009 Vol. 51 No. 2 pp. 13-18zh_CN
dc.identifier.issn0001-5296
dc.identifier.urihttp://www.ib.uj.edu.pl/abc/pdf/51_2/02_ye.pdf
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/10268
dc.description.abstractThis study was aimed at developing an efficient protocol for regeneration of Pseudostellaria heterophylla plantlets and induction of polyploidy. Calli of P. heterophylla (Miq) from stems, leaves and buds as explants could not differentiate into plantlets. However, Young embryo segments used as primary explants produced embryonic calli on MS medium containing 5.0 mg/L 2,4-D and 0.5 mg/L KT. After the embryonic calli with granular protuberances were transferred to MS medium containing 0.5 mg/L BA, they developed shoots and then rooted to form plantlets. Polyploidy was induced when embryonic calli were placed in liquid MS medium containing 0.5% colchicine for 4 days, followed by culturing in solid medium to induce differentiation. Polyploidy was identified by the number of chromosomes and the size of plantlet stomata. The tetraploid plantlets produced larger root tubers than the diploid plantlets.zh_CN
dc.description.sponsorshipNational Natural Science Foundation of China [30670126]; Xiamen Science Item [3502Z20084037]zh_CN
dc.language.isoenzh_CN
dc.publisherPOLSKA AKAD NAUKzh_CN
dc.subjectCalluszh_CN
dc.subjectembryonic callizh_CN
dc.subjectPseudostellaria heterophyllazh_CN
dc.subjectregenerationzh_CN
dc.subjecttetraploidyzh_CN
dc.titleREGENERATION OF PLANTLETS AND TETRAPLOIDY INDUCTION IN PSEUDOSTELLARIA HETEROPHYLLAzh_CN
dc.typeArticlezh_CN


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