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dc.contributor.authorLi, Zhi-Cong
dc.contributor.authorPan, Zhi-Zhen
dc.contributor.authorZhu, Yu-Jing
dc.contributor.authorWang, Qin
dc.contributor.authorChen, Qing-Xi
dc.contributor.author陈清西
dc.contributor.authorChen, Chao-Qi(Peking Univ, Coll Urban & Environm Sci)
dc.contributor.authorYan, Ruo-Rong
dc.contributor.authorYan, Jiang-Hua
dc.date.accessioned2011-07-23T02:14:30Z
dc.date.available2011-07-23T02:14:30Z
dc.date.issued2010
dc.identifier.citationApplied Biochemistry and Biotechnology ,Volume 160, Number 7, 2166-2174zh_CN
dc.identifier.issn0273-2289
dc.identifier.urihttp://dx.doi.org/doi: 10.1007/s12010-009-8714-9
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/10267
dc.description.abstractThe effects of dimethyl sulfoxide (DMSO) on the activity of polyphenol oxidase (PPO, EC 1.14.18.1) from blowfly pupae for the oxidation of L-3,4-dihydroxyphenylalanine were studied. The results showed that low concentrations of DMSO could lead to reversible inactivation to the enzyme. The IC50 value, the inactivator concentration leading to 50% activity lost, was estimated to be 2.35 M. Inactivation of the enzyme by DMSO was classified as mixed type. The kinetics of inactivation of PPO from blowfly pupae in the low concentrations of DMSO solution was studied using the kinetic method of the substrate reaction. The rate constants of inactivation were determined. The results show that k(+0) was much larger than k(+0)', indicating that the free enzyme molecule was more fragile than the enzyme-substrate complex in the DMSO solution. It was suggested that the presence of the substrate offers marked protection of this enzyme against inactivation by DMSO.zh_CN
dc.description.sponsorshipNational High Technology Research and Development Program ("863" Program) of China [2006AA10A212]; Postdoctoral Foundation of China [20080430787]; Science and Technology Foundation of Fujian Province [2007N0051]; Program for Innovative Research Team in Science and Technology in Fujian Province Universityzh_CN
dc.language.isoenzh_CN
dc.publisherHUMANA PRESS INCzh_CN
dc.subjectPolyphenol oxidasezh_CN
dc.subjectBlowfly pupaezh_CN
dc.subjectInactivationzh_CN
dc.subjectKineticszh_CN
dc.subjectDimethyl sulfoxidezh_CN
dc.titleInactivation Kinetics of Polyphenol Oxidase from Pupae of Blowfly (Sarcophaga bullata) in the Dimethyl Sulfoxide Solutionzh_CN
dc.typeArticlezh_CN


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