Cloning and tissue expressions of seven chitinase family genes in Litopenaeus vannamei
Date
2010-07Author
Yan, Jiang-Hua
Huang, Qian-Sheng
Tao, Yi-Ming
Xie, Xiao-Lan
Wang, Ye
Wei, Xiao-Qian
Chen, Qing-Xi
陈清西
Tang, Jian-Yang(Fujian Acad Agr Sci, Agr Bioresource Res Inst)
Yan, Qin-Hua(Fudan Univ, Sch Life Sci)
Collections
- 生命科学-已发表论文 [5901]
Abstract
GH18 chitinase is a multi-gene family The family plays important physiological roles in Crustacea, e g ecdysis and defense against pathogen However, data about GH18 family are rather limited in Crustacea In the study, different cloning strategies were adopted to clone chitinase genes of Litopenaeus vannamei, which is the most widely cultured shrimp Seven chitinase family members were identified. Analysis of domain architectures showed the repeated CBM18 modules and catalytic domain of enzymatically inactive chitolectin in Crustacea for the first time. Comparing to the three known groups of crustacean chitinase, four of the seven members are located on new evolutionary clades thus enriched the chitinase family of Crustacea Tissue expression profiles were investigated in eight tissues Expression of CHT5 and CHID1 were both detected in the hemocyte by which the innate immunity activity was carried out The domain architectures, evolutionary relationships and tissue expression patterns all provide reasonable explanation for the existence of multiple genes in crustacean chitinase family (C) 2010 Elsevier Ltd All rights reserved