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Echinococcus multilocularis: Proteomic analysis of the protoscoleces by two-dimensional electrophoresis and mass spectrometry

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Echinococcus multilocularis Proteomic analysis of the protoscoleces by two-dimensional electrophoresis and mass spectrometry .htm (413bytes)
Date
2009
Author
Wang, Yanhai
王彦海
Cheng, Zhe
程喆
Lu, Xiaofeng
鲁晓风
Tang, Chongti
唐崇惕
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  • 生命科学-已发表论文 [5901]
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Abstract
Echinococcus multilocularis is an important parasite that causes human alveolar echinococcosis. Identification and characterization of the proteins encoded by E. multilocularis metacestode might help to understand the complexity of the parasites and their interactions with the host, and to identify new candidates for immunodiagnosis and vaccine development. Here we present a proteomic analysis of E. multilocularis protoscolex (PSC) proteins. The proteins were resolved by 2-DE (pH range 3.5-10), followed by MALDI-TOF MS analysis. Fourteen known Echinococcus proteins were identified, including cytoskeletal proteins, heat shock proteins, metabolic enzymes, 14-3-3 protein, antigen P-29 and calreticulin. To construct a systematic reference map of the immunogenic proteins from E. multilocularis PSC, immunoblot analysis of PSC 2-DE maps was performed. Over 50 proteins spots were detected on immunoblots as antigens and 15 of them were defined. The results showed that cytoskeletal proteins and heat shock proteins were immunodominant antigens in alveolar echinococcosis. (C) 2009 Elsevier Inc. All rights reserved.
Citation
Experimental Parasitology,Volume 123, Issue 2, October 2009, Pages 162-167
URI
http://dx.doi.org/doi:10.1016/j.exppara.2009.06.014
https://dspace.xmu.edu.cn/handle/2288/10138

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