Three New ent-Trachylobane Diterpenoids from Co-cultures of the Calli of Trewia nudiflora and Fusarium sp WXE

Abstract

Five diterpenoids, including three new ent-trachylobane diterpenoids, i.e., (3 alpha)-3-hydroxy-ent-trachylobane-17,19-dioic acid 19-methyl ester (1), ent-trachylobane-17,19-dioic acid 19-methyl ester (2), ent-trachylobane-17,19-dioic acid (3), and two known atisane-type ones, i.e., (16 alpha)-16,17-dihydroxy-ent-atisan-19-oic acid methyl ester (4), and 17-hydroxy-ent-atisan-19-oic acid methyl ester (5), were isolated from the co-culture extract of the calli of Trewia nudiflora and its endophytic fungus Fusarium sp. WXE. Their structures were elucidated by spectroscopic analyses, including 1D- and 2D-NMR experiments, and HR-Q-TOF mass spectrometry. The antitumor and antibacterial properties of the new compounds were evaluated.