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dc.contributor.authorLi, Qingge
dc.contributor.author李庆阁
dc.contributor.authorRuan, Li
dc.contributor.authorZhao, Huian(Inst Forens Sci, Dept Pub)
dc.date.accessioned2011-07-12T03:44:01Z
dc.date.available2011-07-12T03:44:01Z
dc.date.issued2010
dc.identifier.citationJournal of Forensic Sciences,Volume 55,Issue 1,pages19-24,January 2010zh_CN
dc.identifier.issn0022-1198
dc.identifier.urihttp://dx.doi.org/doi:10.1111/j.1556-4029.2009.01228.x
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/10100
dc.description.abstractRapid and informative ABO genotyping has become increasingly popular in forensic use. We developed a multiplex real-time polymerase chain reaction (PCR) approach to genotype ABO major groups and subgroups. Seven differently fluorophor-labeled displacing probes for O1(261delG), A(261G), A(796C/803C), B(796A/803C), O2 (802G > A), A2 (1059delC), and A2 (1009A > G) were combined in one or two PCRs to determine either ABO major groups or subgroups. The method correctly detected 13 reference DNA samples. A blind test of 237 samples resulted in complete agreement with their phenotypes, and 110 of these 237 samples as well as with PCR-SSP method. The whole analysis could be finished in less than 100 min at substantially low material cost and the template DNA ranging from 0.16 to 500 ng per reaction could be quantitatively detected. Despite the limited informativeness of ABO genotyping, the developed methods could find application in rapid and inexpensive screening of forensic settings.zh_CN
dc.description.sponsorshipedu.cnzh_CN
dc.language.isoenzh_CN
dc.publisherWILEY-BLACKWELL PUBLISHING, INCzh_CN
dc.subjectforensic sciencezh_CN
dc.subjectgenotypingzh_CN
dc.subjectABO blood groupzh_CN
dc.subjectreal-time polymerase chain reactionzh_CN
dc.subjectdisplacing probeszh_CN
dc.subjectsingle nucleotide polymorphismszh_CN
dc.titleMulticolor Real-Time PCR Genotyping of ABO System Using Displacing Probeszh_CN
dc.typeArticlezh_CN


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