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dc.contributor.authorLiang, Yi
dc.contributor.authorWu, Xiaobing
dc.contributor.authorGan, Lihui
dc.contributor.authorXu, Huijuan
dc.contributor.authorHu, Zhong
dc.contributor.authorLong, Minnan
dc.contributor.author龙敏南
dc.date.accessioned2011-07-12T03:19:44Z
dc.date.available2011-07-12T03:19:44Z
dc.date.issued2009
dc.identifier.citationMicrobiological Research,Volume 164, Issue 6, 29 November 2009, Pages 674-679zh_CN
dc.identifier.issn0944-5013
dc.identifier.urihttp://dx.doi.org/doi:10.1016/j.micres.2009.03.001
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/10099
dc.description.abstractHydrogenases are the key enzymes for the biological hydrogen production, which can be classified as H-2-uptake hydrogenase and H-2-production hydrogenase. The genes encoding a membrane-bound [NiFe]-hydrogenase (MBH), which is mainly responsible for hydrogen uptake, from the photosynthetic bacterium Allochromatium vinosum was cloned and sequenced. It consist of two structural genes (hydS, hydL) and two intergenic genes (isp1, isp2), which are therefore organized as hydS-isp1-isp2-hydL. This is different from the arrangement of other typical hydrogenase gene clusters. A deletion mutant-strain Phi hydSL, tacking isp1, isp2, partial hydS and hydL genes, was constructed by marker-exchange mutagenesis. Under dark fermentative conditions, the hydrogen production yield by this mutant increased by 62%. The result suggests that the disruption of MBH could greatly improve the hydrogen production in the cells by decreasing the hydrogen uptake. (C) 2009 Elsevier GmbH. All rights reserved.zh_CN
dc.description.sponsorshipNational 863 project of China [2006AA05Z111]; NSFC National Foundation for Fostering Talents of Basic Science [J0630649, 30470395]zh_CN
dc.language.isoenzh_CN
dc.publisherELSEVIER GMBH, URBAN & FISCHER VERLAGzh_CN
dc.subjectAllochromatium vinosumzh_CN
dc.subjectMembrane-bound hydrogenasezh_CN
dc.subjectGene cloningzh_CN
dc.subjectDeletion of hydrogen-uptake systemzh_CN
dc.subjectBiological hydrogen productionzh_CN
dc.titleIncreased biological hydrogen production by deletion of hydrogen-uptake system in photosynthetic bacteriazh_CN
dc.typeArticlezh_CN


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