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dc.contributor.authorLi, Bowen
dc.contributor.author李博文
dc.contributor.authorWang, Chonggang
dc.contributor.author王重刚
dc.contributor.authorChen, Yixin
dc.contributor.author陈毅歆
dc.contributor.authorZuo, Zhenghong
dc.contributor.author左正宏
dc.contributor.authorYu, Ang
dc.date.accessioned2011-07-12T02:46:53Z
dc.date.available2011-07-12T02:46:53Z
dc.date.issued2010-08-15
dc.identifier.citationAquatic Toxicology,Volume 99, Issue 2, 15 August 2010, Pages 248-255zh_CN
dc.identifier.issn0166-445X
dc.identifier.urihttp://dx.doi.org/doi:10.1016/j.aquatox.2010.05.002
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/10095
dc.description.abstractTributyltin (TBT), a ubiquitous marine environmental contaminant, has been reported to affect functioning of the central nervous system. However, the mechanism of its neurotoxicity remains unknown. In this study, an Anneal Control Primer-differential display Reverse Transcription-PCR method was employed to investigate differentially expressed genes in the brain of Sebastiscus marmoratus in response to acute TBT exposure. A total of 18 gene sequences were identified as having the potential for being differentially expressed, of which 9 could be identified with homologous database sequences. The expression profiles of 4 genes, namely cytochrome c oxidase subunit II, GRB2-associated binding protein 2, adaptor-related protein complex 2, and guanine nucleotide exchange factor p532, were analyzed in the brain using real time fluorescence quantitative PCR after treatment with 10, 100 and 1000 ng/L of TBT for 50 days. The results showed that chronic exposure to TBT induced down-regulation of these genes in a dose dependent manner. The present study provided a basis for studying the response of fish to TBT exposure and allowed the characterization of new potential neurotoxic biomarkers of TBT contamination in seawater. (C) 2010 Elsevier B.V. All rights reserved.zh_CN
dc.description.sponsorshipScience Planning Program of Fujian Province [2009J1010]; National Natural Science Foundation of China [40606027, 20777061]zh_CN
dc.language.isoenzh_CN
dc.publisherELSEVIER SCIENCE BVzh_CN
dc.subjectTributyltinzh_CN
dc.subjectSebastiscus marmoratuszh_CN
dc.subjectACP-ddRT-PCRzh_CN
dc.subjectNeurotoxicityzh_CN
dc.titleIdentification of differentially expressed genes in the brain of Sebastiscus marmoratus in response to tributyltin exposurezh_CN
dc.typeArticlezh_CN


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