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dc.contributor.authorKe, Rongqin
dc.contributor.authorYang, Wei
dc.contributor.authorXia, Xiaohu
dc.contributor.authorXu, Ye
dc.contributor.authorLi, Qingge
dc.contributor.author李庆阁
dc.date.accessioned2011-07-12T01:08:04Z
dc.date.available2011-07-12T01:08:04Z
dc.date.issued2010-11-01
dc.identifier.citationAnalytical Biochemistry,Volume 406, Issue 1, 1 November 2010, Pages 8-13zh_CN
dc.identifier.issn0003-2697
dc.identifier.urihttp://dx.doi.org/doi:10.1016/j.ab.2010.06.039
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/10088
dc.description.abstractWe present a new type of enzyme-antibody conjugate that simplifies the labeling procedure and increases the sensitivity of enzyme-linked immunosorbent assay (ELISA). The conjugates were prepared through layer-by-layer immobilization of enzyme and antibody on a silica nanoparticle scaffold. A maximal amount of enzyme was immobilized on the nanoparticle, followed by antibody linkage through Dextran 500. The conjugate could be easily purified from unreacted reagents by simple centrifugations. In comparison with the conventional antibody-enzyme conjugate used in ELISA, which often has one or two enzyme molecules per antibody, the new type of conjugate contained more enzyme molecules per antibody and provided a much higher signal and increased sensitivity. When used in an ELISA detection of the hepatitis B surface antigen (HBsAg), the detection limit was three times lower than that of the commercially available ELISA kit. (C) 2010 Elsevier Inc. All rights reserved.zh_CN
dc.description.sponsorshipNational Natural Science Foundation of China [30500454]; Natural Science Foundation of Fujian Province of China [2007J0112]zh_CN
dc.language.isoenzh_CN
dc.publisherACADEMIC PRESS INC ELSEVIER SCIENCEzh_CN
dc.subjectELISAzh_CN
dc.subjectSilica nanoparticleszh_CN
dc.subjectTandem conjugationzh_CN
dc.subjectHepatitis B surface antigenzh_CN
dc.titleTandem conjugation of enzyme and antibody on silica nanoparticle for enzyme immunoassayzh_CN
dc.typeArticlezh_CN


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